Electrodiffusion of Lipids on Membrane Surfaces

TL;DR

This paper introduces a variational electrodiffusion model for charged lipids on membrane surfaces, capturing lipid aggregation, saturation, and salt effects, with a finite element method for solving the PDEs.

Contribution

It develops a self-consistent electrodiffusion model incorporating lipid size and membrane geometry, advancing understanding of lipid organization on membranes.

Findings

Model predicts lipid saturation on membrane surfaces.

Demonstrates MARCKS peptide sequesters PIP2 lipids.

Shows salt concentration affects lipid sequestration.

Abstract

Random lateral translocation of lipids and proteins is a universal process on membrane surfaces. Local aggregation or organization of lipids and proteins can be induced when this lateral random diffusion is mediated by the electrostatic interactions and membrane curvature. Though the lateral diffusion rates of lipids on membrane of various compositions are measured and the electrostatic free energies of predetermined protein-membrane-lipid systems can be computed, the process of the aggregation and the evolution to the electrostatically favorable states remain undetermined. Here we propose an electrodiffusion model, based on the variational principle of free energy functional, for the self-consistent lateral drift-diffusion of multiple species of charged lipids on membrane surfaces. Finite sizes of lipids are modeled to enforce the geometrical constraint of the lipid concentration on membrane surfaces. A surface finite element method is developed to appropriate the Laplace-Beltrami operators in the partial differential equations (PDEs) of the model. Our model properly describes the saturation of lipids on membrane surface, and correctly predicts that the MARCKS peptide can consistently sequester three multivalent phosphatidylinositol 4,5-bisphosphate (\pip2) lipids through its basic amino acid residues, even there is a large fraction of monovalent phosphatidylserine (PS) in the membrane. Solutions of the PDEs also show the salt-dependence of the lipid sequestration.