Mechanisms for covalent immobilization of horseradish peroxi-dase on ion beam treated polyethylene

TL;DR

This study investigates how ion beam treatment modifies polyethylene surfaces to enable covalent immobilization of horseradish peroxidase, maintaining enzyme activity through free radical reactions and providing a universal attachment mechanism.

Contribution

It reveals a covalent attachment mechanism via surface free radicals, applicable to various proteins and radiation-damaged polymers, with long-term stability of enzyme activity.

Findings

Surface modification increases free radicals and surface energy.

Covalent attachment retains enzymatic activity for over 2 years.

Universal mechanism involves free radicals reacting with amino acids.

Abstract

The mechanism that provides the observed strong binding of biomolecules to polymer sur-faces modified by ion beams is investigated. The surface of polyethylene (PE) was modified by plasma immersion ion implantation with nitrogen ions. Structure changes including car-bonization and oxidation were observed in the modified surface layer of PE by Raman spec-troscopy, FTIR ATR spectroscopy, atomic force microscopy, surface energy measurement and XPS spectroscopy. An observed high surface energy of the modified polyethylene was attributed to the presence of free radicals on the surface. The surface energy decay with stor-age time after PIII treatment was explained by a decay of the free radical concentration while the concentration of oxygen-containing groups increased with storage time. Horseradish per-oxidase was covalently attached onto the modified PE surface. The enzymatic activity of co-valently attached protein remained high. A mechanism based on the covalent attachment by the reaction of protein with free radicals in the modified surface is proposed. Appropriate blocking agents can block this reaction. All aminoacid residues can take part in the covalent attachment process, providing a universal mechanism of attachment for all proteins. The long-term activity of the modified layer to attach protein (at least 2 years) is explained by stabilisa-tion of unpaired electrons in sp2 carbon structures. The native conformation of attached pro-tein is retained due to hydrophilic interactions in the interface region. A high concentration of free radicals on the surface can give multiple covalent bonds to the protein molecule and de-stroy the native conformation and with it the catalytic activity. The universal mechanism of protein attachment to free radicals could be extended to various methods of radiation damage of polymers.