Quantifying the Reversible Association of Thermosensitive Nanoparticles

TL;DR

This paper presents a theoretical model combined with light-scattering experiments to quantify temperature-dependent association and dissociation rates of thermosensitive nanoparticles, revealing sharp changes in binding energy linked to hydrophobic-hydrophilic transitions.

Contribution

It introduces a novel method to measure microscopic binding rates and energies for thermosensitive nanoparticles as a function of temperature.

Findings

Binding energy varies sharply with temperature.

Reversible aggregation is driven by hydrophobic-hydrophilic transition.

Method successfully applied to polystyrene/poly(N-isopropylacrylamide) nanoparticles.

Abstract

Under many conditions, biomolecules and nanoparticles associate by means of attractive bonds, due to hydrophobic attraction. Extracting the microscopic association or dissociation rates from experimental data is complicated by the dissociation events and by the sensitivity of the binding force to temperature (T). Here we introduce a theoretical model that combined with light-scattering experiments allows us to quantify these rates and the reversible binding energy as a function of T. We apply this method to the reversible aggregation of thermoresponsive polystyrene/poly(N-isopropylacrylamide) core-shell nanoparticles, as a model system for biomolecules. We find that the binding energy changes sharply with T, and relate this remarkable switchable behavior to the hydrophobic-hydrophilic transition of the thermosensitive nanoparticles.