Fluorescence spectrum analysis using Fourier series modeling for Fluorescein solution in Ethanol

TL;DR

This study models the fluorescence spectrum of fluorescein in ethanol across various temperatures using Fourier series, demonstrating the method's effectiveness in fitting experimental data and predicting spectra.

Contribution

Introduces Fourier series modeling as an effective approach for analyzing fluorescence spectra of fluorescein in ethanol at different temperatures.

Findings

Fourier series (3×2) best fits the fluorescence spectra.

Good agreement between theoretical and experimental spectra at 183K.

Temperature-dependent spectral analysis demonstrated successfully.

Abstract

We have measured the fluorescence spectrum for fluorescein solution in ethanol with concentration 1 {\times} 10-3 mol/liter at different temperatures from room temperature to freezing point of solvent, (T = 153, 183, 223, 253, and 303 K) using liquid nitrogen. Table curve 2D version 5.01 program has been used to determine the fitting curve and fitting equation for each fluorescence spectrum. Fourier series (3 {\times} 2) was the most suitable fitting equation for all spectra. Theoretical fluorescence spectrum of fluorescein in ethanol at T = 183K was calculated and compared with experimental fluorescence spectrum at the same temperature. There is a good similarity between them.