Quantum yield optimized fluorophores for site-specific labeling and super-resolution imaging

TL;DR

This paper introduces a new method for creating highly bright and stable fluorophores with optimized quantum yield, enabling improved site-specific labeling and super-resolution imaging, addressing limitations of existing dyes and quantum dots.

Contribution

The authors develop a novel fluorophore design with enhanced quantum yield for precise, stable, and site-specific labeling in super-resolution microscopy, overcoming current limitations of dye size and biocompatibility.

Findings

Achieved higher quantum yield in fluorophores

Enabled site-specific labeling without large quantum dots

Improved stability and brightness for super-resolution imaging

Abstract

Single molecule applications, saturated pattern excitation microscopy, or stimulated emission depletion (STED) microscopy demand for bright and highly stable fluorescent dyes1,2. Despite of intensive research the choice of fluorphores is still very limited. Typically a stable fluorescent dyes is covalently attached to the target. This methodology brings forward a number of limitations, in particular, in case of protein labeling. First of all the fluorescent probes need to be attached selectively and site-specifically to prevent unspecific background. This often requires single cysteine mutations for covalent protein modification. Employing quantum dots allows overcoming problems of photo-bleaching3-6. However, the downsides are their large size, rendering the probe inaccessible to spatially confined architectures, issues in biocompatibility due to proper particle coating, and cellular toxicity6-8. Here we propose a new method to overcome the above outlined problems.