Spindles and active vortices in a model of confined filament-motor mixtures

TL;DR

This study uses numerical simulations to explore how filament-motor interactions lead to various self-organized structures like spindles, asters, and vortices in confined active gels, revealing the influence of parameters on these patterns.

Contribution

It provides a detailed numerical analysis of filament-motor systems in confinement, elucidating the emergence and dynamics of complex structures and their dependence on motor activity and pressure.

Findings

Stable spindles, asters, and vortices spontaneously form in simulations.

Vortex lifetimes follow a Poisson process, indicating stochastic contraction.

Increasing motor detachment rate can destroy vortices and convert asters into vortices.

Abstract

Robust self-organization of subcellular structures is a key principle governing the dynamics and evolution of cellular life. In fission yeast cells undergoing division, the mitotic spindle spontaneously emerges from the interaction of microtubules, motor proteins and the confining cell walls, and asters and vortices have been observed to self-assemble in quasi-two dimensional microtubule-kinesin assays. Their is no clear microscopic picture of the role of the active motors driving this pattern formation, and the relevance of continuum modeling to filament-scale structures remains uncertain. Here we present results of numerical simulations of a discrete filament-motor protein model confined to a pressurised cylindrical box. Stable spindles, nematic configurations, asters and high-density semi-asters spontaneously emerge, the latter pair having also been observed in cytosol confined within emulsion droplets. State diagrams are presented delineating each stationary state as the pressure, motor speed and motor density are varied. We further highlight a parameter regime where vortices form exhibiting collective rotation of all filaments, but have a finite life-time before contracting to a semi-aster. Quantifying the distribution of life-times suggests this contraction is a Poisson process. Equivalent systems with fixed volume exhibit persistent vortices with stochastic switching in the direction of rotation, with switching times obeying similar statistics to contraction times in pressurised systems. Furthermore, we show that increasing the detachment rate of motors from filament plus-ends can both destroy vortices and turn some asters into vortices. Based on our findings we argue the need for a deeper understanding of the microscopic activities underpinning macroscopic self-organization in active gels and urge further experiments to help bridge these lengths.