Quantitative methods for deconvolution of true topographical properties of object on the basis of measured AFM-images: Part 2. Broadening effect

TL;DR

This paper develops a quantitative AFM image deconvolution technique to accurately determine true topographical properties of objects, demonstrated on DNA-surfactant complexes.

Contribution

It introduces a two-parameter model to correct for image broadening effects in AFM, enabling precise measurement of molecular structures.

Findings

Successfully restored real geometrical parameters of objects

Provided quantitative insights into DNA-surfactant molecular structures

Enhanced accuracy of AFM topographical analysis

Abstract

Technique of the quantitative description of image broadening effect in AFM allowing to restore real geometrical parameters of object using two-parameters model (height and width at half-height) is developed. Application of the technique has allowed to receive the quantitative information about molecular structure of DNA-surfactant complexes.