Properties of IA in a serotonergic neuron of the dorsal raphe nucleus

TL;DR

This study characterizes the properties of the IA potassium current in serotonergic neurons of the rat dorsal raphe nucleus using voltage clamp data and introduces a novel method for analyzing activation functions.

Contribution

It provides detailed biophysical parameters of IA in DRN serotonergic neurons and presents a new method for accurately determining activation functions.

Findings

Maximal conductance g=20.5 nS

Half-activation potential V_a=-52.5 mV

Half-inactivation potential V_i=-91.5 mV

Abstract

Voltage clamp data were analyzed in order to characterize the properties of the fast potassium transient current IA for a serotonergic neuron of the rat dorsal raphe nucleus (DRN). We obtain maximal conductance, time constants of activation and inactivation, and the steady state activation and inactivation functions, as Boltzmann curves, defined by half-activation potentials and slope factors. We employ a novel method to accurately obtain the activation function and compare the results with those obtained by other methods. The form of IA is estimated as g(V-V_{rev}) m^4h with g=20.5 nS. For activation, the half-activation potential is V_a=-52.5 mV with slope factor k_a=16.5 mV, whereas for inactivation the corresponding quantities are -91.5 mV and -9.3 mV. We discuss the results in terms of the corresponding properties of \IA in other cell types and their possible relevance to pacemaking activity in 5-HT cells of the DRN.