Study of the combined effect of temperature, pH and water activity on the radial growth rate of the white-rot basidiomycete Physisporinus vitreus by using a hyphal growth model

TL;DR

This study uses a discrete hyphal growth model to analyze how temperature, pH, and water activity influence the growth rate of P. vitreus, providing insights for biotechnological applications like bioincising.

Contribution

It introduces a lattice-free discrete modeling approach to connect microscopic hyphal growth costs with macroscopic fungal colony expansion under environmental factors.

Findings

Growth rate follows a power law related to environmental conditions.

Model aligns well with laboratory experiments.

Insights into fungal colonization strategies in wood substrates.

Abstract

The present work investigates environmental effects on the growth of fungal colonies of P. vitreus by using a lattice-free discrete modelling approach called FGM (Fuhr et al. (2010), arXiv:1101.1747), in which hyphae and nutrients are considered as discrete structures. A discrete modelling approach allows studying the underlying mechanistic rule concerning the basic architecture and dynamic of fungal networks on the scale of a single colony. By comparing simulations of the FGM with laboratory experiments of growing fungal colonies on malt extract agar we show that combined effect of temperature, pH and water activity on the radial growth rate of a fungal colony on a macroscopic scale may be explained by a power law for the growth costs of hyphal expansion on a microscopic scale. The information about the response of the fungal mycelium on a microscopic scale to environmental conditions is essential to simulate its behavior in complex structure substrates such as wood, where the impact of the fungus to the wood (i.e. the degradation of bordered pits or the creation of bore holes and cavities) changes the local environmental condition, e.g. the permeability of the substrate and therefore the water activity level in a colonized wood cell lumen. A combination of diffusion and moisture processes into wood with the FGM may brighten the knowledge about the colonization strategy of P. vitreus and helps to optimize its growth behavior for biotechnological application such as bioincising.