Heat shock partially dissociates the overlapping modules of the yeast protein-protein interaction network: a systems level model of adaptation

TL;DR

This study models how heat shock causes partial disintegration of yeast protein interaction networks, revealing a system-level adaptation mechanism that may be common across various complex systems during stress.

Contribution

It introduces a systems-level model showing how heat shock reorganizes the yeast interactome, highlighting module disintegration and structural transition during stress response.

Findings

Heat shock decreases module overlaps and connections in yeast interactome.

The weighted diameter of the interactome increases 4.9-fold under heat shock.

Structural transition resembles a shift from global connectivity to multifocal organization.

Abstract

Network analysis became a powerful tool in recent years. Heat shock is a well-characterized model of cellular dynamics. S. cerevisiae is an appropriate model organism, since both its protein-protein interaction network (interactome) and stress response at the gene expression level have been well characterized. However, the analysis of the reorganization of the yeast interactome during stress has not been investigated yet. We calculated the changes of the interaction-weights of the yeast interactome from the changes of mRNA expression levels upon heat shock. The major finding of our study is that heat shock induced a significant decrease in both the overlaps and connections of yeast interactome modules. In agreement with this the weighted diameter of the yeast interactome had a 4.9-fold increase in heat shock. Several key proteins of the heat shock response became centers of heat shock-induced local communities, as well as bridges providing a residual connection of modules after heat shock. The observed changes resemble to a "stratus-cumulus" type transition of the interactome structure, since the unstressed yeast interactome had a globally connected organization, similar to that of stratus clouds, whereas the heat shocked interactome had a multifocal organization, similar to that of cumulus clouds. Our results showed that heat shock induces a partial disintegration of the global organization of the yeast interactome. This change may be rather general occurring in many types of stresses. Moreover, other complex systems, such as single proteins, social networks and ecosystems may also decrease their inter-modular links, thus develop more compact modules, and display a partial disintegration of their global structure in the initial phase of crisis. Thus, our work may provide a model of a general, system-level adaptation mechanism to environmental changes.