The probability analysis of opening of DNA

TL;DR

This paper analyzes how DNA double helix opening depends on sequence, temperature, force, and experimental constraints, revealing differences between force-induced and thermal melting.

Contribution

It provides a probabilistic analysis of DNA opening considering sequence and physical constraints, highlighting differences in melting behavior under various conditions.

Findings

Force-induced melting differs from thermal melting with constrained ends.

Opening probability depends on sequence and experimental constraints.

Constraints significantly influence DNA melting behavior.

Abstract

We have studied the separation of a double stranded DNA (dsDNA), which is driven either by the temperature or force. By monitoring the probability of opening of entire base pairs along the chain, we show that the opening of a dsDNA depends not only on the sequence but also on the constraints on the chain in the experimental setups. Our results clearly demonstrate that the force induced melting of dsDNA, whose one of the ends is constrained, is significantly different from the thermal melting, when both ends free.