A General Theory for Direct Quantitative Analysis of Antigen

TL;DR

This paper introduces a general theoretical framework for directly quantifying antigens through a potential homogeneous immunoreaction system, providing an equation to determine antigen concentration from complex formation, validated with experimental data.

Contribution

It presents a novel theoretical model for direct antigen analysis and demonstrates its practical applicability with experimental validation using a commercial immunoassay kit.

Findings

The theory accurately predicts antigen concentration from complex concentration profiles.

Experimental results align with theoretical predictions, confirming practical applicability.

The model simplifies antigen quantification in immunoassays.

Abstract

A theory for direct quantitative analysis of an antigen is proposed. It is based on a potential homogenous immunoreaction system. It establishes an equation to describe the concentration change of the antigen and antibody complex. A maximum point is found in the concentration profile of the complex which can be used to calculate the concentration of the antigen. An experimental scheme was designed for a commercial time-resolved fluoroimmunoassay kit for HBsAg, which is based heterogeneous immunoreaction. The results showed that the theory is practically applicable.