Influence of proteins from physiological solutions on the electrochemical behaviour of the Ti-6Al-4V alloy: reproducibility and time-frequency dependence. ---- Influence de la teneur en prot\'eines de solutions physiologiques sur le comportement \'electrochimique du Ti-6Al-4V : reproductibilit\'e et repr\'esentation temps-fr\'equence

TL;DR

This study investigates how proteins in physiological solutions affect the electrochemical behavior of Ti-6Al-4V alloy, using impedance spectroscopy, revealing solution-dependent protection levels and adsorption signatures, with implications for biomedical implant corrosion.

Contribution

It provides new insights into the reproducibility and time-frequency characteristics of Ti-6Al-4V in various physiological media, highlighting the impact of proteins and antioxidants on corrosion behavior.

Findings

Deionised water offers the best protection for Ti-6Al-4V.

PBS with albumin provides the least protection.

Time-frequency graphs reveal adsorption signatures of organic and inorganic species.

Abstract

The electrochemical behaviour of the biomedical and metallic alloys, especially in the orthopaedic implants fields, raises many questions. This study is dedicated for studying the Ti-6Al-4V alloy, by electrochemical impedance spectroscopy, EIS, in various physiological media,: Ringer solution, phosphate buffered solution (PBS), PBS solution and albumin, PBS solution with calf serum and PBS solution with calf serum and an antioxidant (sodium azide). Moreover, the desionised water was considered as the reference solution. The tests reproducibility was investigated. The time-frequency-Module graphs highlighted that the desionised water is the most protective for the Ti-6Al-4V alloy. This biomedical alloy is the less protected in the solution constituted by PBS and albumin. The time-frequency graph allows pointing out the graphic signatures of adsorption for organic and inorganic species (differences between the modules means in studied solution and the modules mean in the reference solution). --- Le comportement \'electrochimique des alliages m\'etalliques biom\'edicaux, notamment dans le domaine des implants orthop\'ediques, pose encore de nombreuses questions. Ce travail propose d'\'etudier l'alliage de titane Ti-6Al-4V, par spectroscopie d'imp\'edance \'electrochimique, SIE, dans diff\'erents milieux physiologiques : solution de Ringer, solution \`a base d'un tampon phosphate (PBS), solution PBS avec de l'albumine, solution PBS avec du s\'erum bovin et une solution PBS avec du s\'erum bovin et un antioxydant (azoture de sodium). De plus, une solution d'eau ultra-pure servira de r\'ef\'erence. La reproductibilit\'e des tests a \'et\'e \'etudi\'ee. Les repr\'esentations temps-fr\'equence des modules ont mis en \'evidence que l'eau d\'esionis\'ee est la solution qui pr\'esente le caract\`ere le plus protecteur pour le Ti-6Al-4V. Cet alliage de titane est le moins prot\'eg\'e dans la solution de PBS contenant de l'albumine. Cette repr\'esentation permet de mettre en \'evidence des signatures graphiques d'adsorption des esp\`eces inorganiques et organiques (diff\'erences entre les moyennes des modules dans les solutions \'etudi\'ees et la moyenne des modules dans la solution de r\'ef\'erence).