Coherent Nonlinear Single Molecule Microscopy

TL;DR

This paper introduces a nonlinear localization microscopy technique based on Rabi oscillations, achieving subwavelength resolution and improved accuracy over traditional methods, with potential for faster imaging and broader applicability.

Contribution

The paper presents a novel nonlinear microscopy method utilizing Rabi oscillations for enhanced localization accuracy and multi-emitter imaging capabilities.

Findings

Achieved subwavelength features smaller than λ/10 in cryogenic experiments.

Monte Carlo simulations show superior localization accuracy compared to linear schemes.

Potential for higher speed and lower photon requirements in imaging.

Abstract

We investigate a nonlinear localization microscopy method based on Rabi oscillations of single emitters. We demonstrate the fundamental working principle of this new technique using a cryogenic far-field experiment in which subwavelength features smaller than $\lambda$/10 are obtained. Using Monte Carlo simulations, we show the superior localization accuracy of this method under realistic conditions and a potential for higher acquisition speed or a lower number of required photons as compared to conventional linear schemes. The method can be adapted to other emitters than molecules and allows for the localization of several emitters at different distances to a single measurement pixel.