UVB radiation induced effects on cells studied by FTIR spectroscopy

TL;DR

This study investigates UVB radiation effects on Jurkat cells using FTIR spectroscopy and flow cytometry, revealing spectral changes associated with apoptosis and cellular biomolecular alterations.

Contribution

It combines FTIR spectroscopy with flow cytometry to identify spectral markers of apoptosis induced by UVB in cancer cells, providing a novel non-invasive analysis method.

Findings

Significant spectral changes in protein and nucleic acid regions after UVB exposure.

Identification of potential FTIR markers correlated with apoptosis.

Evidence of UVB-induced biomolecular alterations in tumor cells.

Abstract

We have made a preliminary analysis of the results about the eVects on tumoral cell line (lymphoid T cell line Jurkat) induced by UVB radiation (dose of 310 mJ/cm^2) with and without a vegetable mixture. In the present study, we have used two techniques: Fourier transform infrared spectroscopy (FTIR) and flow cytometry. FTIR spectroscopy has the potential to provide the identiWcation of the vibrational modes of some of the major compounds (lipid, proteins and nucleic acids) without being invasive in the biomaterials. The second technique has allowed us to perform measurements of cytotoxicity and to assess the percentage of apoptosis. We already studied the induction of apoptotic process in the same cell line by UVB radiation; in particular, we looked for correspondences and correlations between FTIR spetroscopy and flow cytometry data finding three highly probable spectroscopic markers of apoptosis (Pozzi et al. in Radiat Res 168:698-705, 2007). In the present work, the results have shown significant changes in the absorbance and spectral pattern in the wavenumber protein and nucleic acids regions after the treatments.