UVB-Radiation-Induced Apoptosis in Jurkat Cells: A Coordinated Fourier Transform Infrared Spectroscopy-Flow Cytometry Study

TL;DR

This study combines FTIR spectroscopy and flow cytometry to identify spectroscopic markers of UVB-induced apoptosis in Jurkat cells, revealing correlations between spectral changes and apoptotic cell populations.

Contribution

It introduces a combined FTIR and flow cytometry approach to detect apoptosis markers, providing new insights into cell modifications during apoptosis.

Findings

Identified three spectroscopic markers correlated with apoptosis stages.

Wave number shifts of Amide I beta-sheet and 1083 cm^-1 band track early apoptosis.

Amide I area correlates with both early and late apoptosis.

Abstract

We studied the induction of apoptosis in Jurkat cells by UVB radiation (wavelength 290-320 nm) at a dose of 310 mJ/cm^2. We combined Fourier transform infrared (FTIR) spectroscopy with flow cytometry to determine whether the combination of both techniques could provide new and improved information about cell modifications. To do this, we looked for correspondences and correlations between spectroscopy and flow cytometry data and found three highly probable spectroscopic markers of apoptosis. The behavior of the wave number shift of both the Amide I beta-sheet component and the area of the 1083 cm^-1 band reproduced, with a high correlation, the behavior of the early apoptotic cell population, while the behavior of the Amide I area showed a high correlation with the early plus late apoptotic cell population.