Mapping cellular magnesium using X-ray microfluorescence and atomic force microscopy
Stefano Lagomarsino, Stefano Iotti, Giovanna Farruggia, Alessia, Cedola, Valentina Trapani, Michela Fratini, Inna Bukreeva, Andrea, Notargiacomo, Lucia Mastrototaro, Ian McNulty, Stefan Vogt, Daniel Legnini,, Sangsoo Kim, Jeanette A M Maier, Federica I Wolf
TL;DR
This paper introduces a novel method combining X-ray microfluorescence and atomic force microscopy to map intracellular magnesium with high spatial resolution in individual cells.
Contribution
It presents an innovative experimental approach that integrates elemental and morphological data to localize magnesium within cells at high resolution.
Findings
Successful generation of magnesium concentration maps in cells
Integration of X-ray fluorescence and AFM provides detailed cellular magnesium distribution
Method enables precise localization of magnesium in dehydrated mammary epithelial cells
Abstract
Magnesium is the most abundant intracellular divalent cation. We present an innovative experimental approach to localizing intracellular magnesium that combines elemental and morphological information from individual cells with high-resolution spatial information. Integration of information from scanning fluorescence X-ray microscopy with information from atomic force microscopy was used to generate a magnesium concentration map and to determine the X-ray linear absorption coefficient map within a whole dehydrated mammary epithelial cell.
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Taxonomy
TopicsDermatological and Skeletal Disorders · Magnesium in Health and Disease