Technical performance and interpretation of physical experiment in problems of cell biology

TL;DR

This paper reviews recent advances in designing and interpreting physical experiments in cell biology, emphasizing stochastic systems theory, microscopy innovations, and modeling approaches for better understanding living cells.

Contribution

It introduces a theoretical framework based on stochastic systems theory, discusses experimental design, and proposes new microscopy methods and models for cell analysis.

Findings

Light microscopy can achieve resolution comparable to electron microscopy.

Objective analysis methods for cell behavior are feasible.

A new microscope design combining advantages of existing methods is proposed.

Abstract

The lecture summarises main results of my team over last five years in the field of technical experiment design and interpretation of results of experiments for cell bi-ology. I introduce the theoretical concept of the experiment, based mainly on ideqas of stochastic systems theory, and confront it with general ideas of systems theory. In the next part I introduce available experiments and discuss their information con-tent. Namely, I show that light microscopy may be designed to give resolution com-parable to that of electron microscopy and that may be used for experiments using living cells. I show avenues to objective analysis of cell behavior observation. I pro-pose new microscope design, which shall combine advantages of all methods, and steps to be taken to build a model of living cells with predictive power for practical use