Optical tweezers: wideband microrheology

TL;DR

This paper introduces a simple experimental method using optical tweezers to measure the viscoelastic properties of complex fluids across a broad frequency spectrum, addressing limitations of previous techniques.

Contribution

It presents a novel microrheological technique that extends optical tweezers measurements to cover a wider frequency range by employing a generalized Langevin equation.

Findings

Achieved wideband frequency measurements of complex fluids.

Demonstrated consistency with existing high- and low-frequency data.

Provided a practical approach for comprehensive microrheology.

Abstract

Microrheology is a branch of rheology having the same principles as conventional bulk rheology, but working on micron length scales and micro-litre volumes. Optical tweezers have been successfully used with Newtonian fluids for rheological purposes such as determining fluid viscosity. Conversely, when optical tweezers are used to measure the viscoelastic properties of complex fluids the results are either limited to the material's high-frequency response, discarding important information related to the low-frequency behavior, or they are supplemented by low-frequency measurements performed with different techniques, often without presenting an overlapping region of clear agreement between the sets of results. We present a simple experimental procedure to perform microrheological measurements over the widest frequency range possible with optical tweezers. A generalised Langevin equation is used to relate the frequency-dependent moduli of the complex fluid to the time-dependent trajectory of a probe particle as it flips between two optical traps that alternately switch on and off.