Complete Human Mitochondrial Genome Construction Using L-systems

TL;DR

This paper introduces an L-system-based mathematical approach to construct and potentially edit long DNA sequences, demonstrated by generating the entire human mitochondrial genome using standard PC computing power.

Contribution

The paper presents a novel application of L-systems for DNA sequence generation and editing, enabling the synthesis of entire human mitochondrial genomes with accessible computational resources.

Findings

Successfully generated the complete human mitochondrial genome using L-systems.

Demonstrated the potential for editing and correcting DNA sequences with this mathematical model.

Showed that large DNA sequences can be constructed with ordinary PC power.

Abstract

Recently, scientists from The Craig J. Venter Institute reported construction of very long DNA molecules using a variety of experimental procedures adopting a number of working hypotheses. Finding a mathematical rule for generation of such a long sequence would revolutionize our thinking on various advanced areas of biology, viz. evolution of long DNA chains in chromosomes, reasons for existence of long stretches of non-coding regions as well as would usher automated methods for long DNA chains preparation for chromosome engineering. However, this mathematical principle must have room for editing / correcting DNA sequences locally in those areas of genomes where mutation and / or DNA polymerase has introduced errors over millions of years. In this paper, we report the basics and applications of L-system (a mathematical principle) which could answer all the aforesaid issues. At the end, we present the whole human mitochondrial genome which has been generated using this mathematical principle using PC computation power. We can claim now that we can make any stretch of DNA, be it 936 bp of olfactory receptor, with or without introns, mitochondrial DNA to 3 x 109 bp DNA sequences of the whole human genome with even a PC computation power.