# MPAthic: Quantitative Modeling of Sequence-Function Relationships for massively parallel assays

## TL;DR

MPAthic is a new software tool that improves the analysis of DNA, RNA, and protein sequence-function relationships from large-scale experiments.

## Contribution

MPAthic introduces a novel method for accurately quantifying epistatic interactions in massively parallel assays.

## Key findings

- MPAthic outperforms existing software in modeling sequence-function relationships.
- MPAthic can accurately quantify epistatic interaction strengths.
- The tool works with both simulated and published MPA datasets.

## Abstract

Massively parallel assays (MPAs) are being rapidly adopted for studying a wide range of DNA, RNA, and protein sequence-function relationships. However, the software available for quantitatively modeling these relationships is severely limited. Here we describe MPAthic, a software package that enables the rapid inference of such models from a variety of MPA datasets. Using both simulated and previously published data, we show that the modeling capabilities of MPAthic greatly improve on those of existing software. In particular, only MPAthic can accurately quantify the strength of epistatic interactions. These capabilities address a major need in the analysis of MPA data.