# CHITOSAN FROM Portunus Pelagicus IN THE SYNTHESIS OF REDUCED GOLD NANOPARTICLE AS POTENTIAL CARRIER FOR THE DELIVERY OF ERYTHROPOIETIN

## TL;DR

This study uses chitosan from Portunus Pelagicus to create gold nanoparticles that can deliver erythropoietin more effectively.

## Contribution

A novel method for synthesizing gold nanoparticles using chitosan from Portunus Pelagicus for drug delivery is introduced.

## Key findings

- Chitosan serves as a reducing agent, stabilizer, and penetration enhancer in gold nanoparticle synthesis.
- Erythropoietin shows high loading efficiency with chitosan-reduced gold nanoparticles via hydrogen bonding.
- The nanoparticles improve erythropoietin's pharmacodynamics and mucosal cellular uptake.

## Abstract

Nanotechnology and its promises for clinical translation to targeted drug delivery with limited accompanying toxicity provide exciting research opportunities that demands multidisciplinary approaches. The colloidal metallic systems have been recently investigated in the area of nanomedicine. Gold nanoparticles have found themselves useful for diagnostics and drug delivery applications. In this study, we have reported a novel method for the synthesis of gold nanoparticles using natural, biocompatible and biodegradable chitosan which came from deacetylating chitin from Portunus Pelagicus. It serves many purposes, as a reducing agent, stabilizer and absorption and penetration enhancer.

Erythropoietin would have high loading efficiency with chitosan reduced gold nanoparticles; the binding is predominantly through hydrogen bonding. Chitosan reduced gold nanoparticles improve the pharmacodynamics and cellular uptake of Erythropoietin across mucosal sites and have immunoadjuvant properties.

There is almost 50 % shell waste in crustacean industry. It is resourceful if it would be bioconverted. The process of bioconversion is deproteination, demineralization and deacetylation to obtain chitosan. In synthesizing gold nanoparticles, 1.48 × 10−2 M chloroauric acid will be reduced by heating for 15 minutes in 100mL chitosan solution prepared in 1% acetic acid to yield a ruby-red solution. Erythropoietin would be loaded into it and will undergo 13,000rpm of centrifuge followed by calculating the loading efficiency.