# Ewing sarcoma breakpoint region 1 prevents transcription-associated genome instability

## TL;DR

This study shows that EWSR1, an RNA-binding protein, helps prevent DNA damage in its own gene region, which could be important in cancer development.

## Contribution

The study reveals a new role for EWSR1 in preventing DNA double-strand breaks in its own locus.

## Key findings

- Reduced EWSR1 expression increases DNA double strand breaks in its translocation-prone region.
- EWSR1 binds to intronic regions in polyadenylated nuclear RNAs, including its own locus.
- EWSR1's RNA-binding activity may be linked to genomic instability and cancer-related translocations.

## Abstract

Ewing Sarcoma break point region 1 (EWSR1) is a multi-functional RNA-binding protein that is involved in many cellular processes, from gene expression to RNA processing and transport. Translocations into its locus lead to chimeric proteins with tumorigenic activity, that lack the RNA binding domain. With crosslinking and immunoprecipitation we have found that EWSR1 binds to intronic regions that are present in polyadenylated nuclear RNAs, which include the translocation-prone region of its own locus. Reduced EWSR1 expression leads to gene expression changes that indicate reduced proliferation. By fluorescence in situ hybridization (FISH) with break-apart probes that flanked the translocation-prone region within the EWSR1 locus we found that reduced EWSR1 expression increases the frequency of split signals, indicative of DNA double strand breaks (DSB). The response in phosphorylated histone H2AX and p53-binding protein 1 (53BP1) double-stained foci to the topoisomerase poison camptothecin in cells treated with a control shRNA and with sh-EWSR1 further suggests that EWSR1 functions in the prevention of DNA DSBs. Our data reveal a new function of the EWSR1 member of the FET family and suggest a connection between the RNA-binding activity of EWSR1 and the instability of its own locus that may play a role in malignancy-associated translocations.