# TransRate: reference free quality assessment of de-novo transcriptome assemblies

## TL;DR

TransRate is a tool that evaluates the quality of newly assembled transcriptomes without needing a reference genome.

## Contribution

TransRate introduces a novel reference-free method for assessing transcriptome assembly quality using only sequenced reads.

## Key findings

- TransRate accurately evaluates assemblies of conserved and novel RNA molecules across species.
- TransRate outperforms comparable methods in accuracy for transcriptome quality assessment.

## Abstract

TransRate is a tool for reference-free quality assessment of de novo transcriptome assemblies. Using only sequenced reads as the input, TransRate measures the quality of individual contigs and whole assemblies, enabling assembly optimization and comparison. TransRate can accurately evaluate assemblies of conserved and novel RNA molecules of any kind in any species. We show that it is more accurate than comparable methods and demonstrate its use on a variety of data.