Infrared Surface-Plasmon-Resonance -- a novel biophysical tool for studying living cell

TL;DR

This paper introduces infrared surface plasmon resonance (IR-SPR) as a sensitive biophysical tool for studying living cells, demonstrating its advantages over traditional visible-range SPR in biological applications.

Contribution

It presents the development and application of FTIR-SPR, highlighting its higher sensitivity and versatility for real-time biological studies in aqueous environments.

Findings

IR-SPR sensitivity is higher than visible-range SPR.

FTIR-SPR can monitor glucose concentration and uptake.

IR-SPR enables studying cell membrane interactions.

Abstract

We discuss the Surface-Plasmon-Resonance (SPR) technique based on Fourier -Transform - InfraRed (FTIR) spectrometry. We explore the potential of the infrared surface plasmon resonance technique for biological studies in aqueous solutions and compare it to the conventional surface plasmon technique operating in the visible range. We demonstrate that the sensitivity of the SPR technique in the infrared range is not lower and in fact is even higher. We show several examples of applying FTIR-SPR for biological studies: (i) monitoring D-glucose concentration in solution, and (ii) measuring D-glucose uptake by erythrocytes in suspension. We emphasize the advantages of infrared SPR for studying living cell cultures and show how this technique can be used for characterization of (i) cholesterol penetration into plasma membrane, and (ii) transferrin-induced clathrin-mediated endocytosis.