Terahertz response of dipolar impurities in polar liquids: On anomalous dielectric absorption of protein solutions

TL;DR

This paper presents a new theoretical model for terahertz absorption in dielectric mixtures, explaining anomalous protein solution absorption by considering mutual polarization effects and hydration shell polarization.

Contribution

The study introduces a coarse-grained nonlinear theory for terahertz absorption that accounts for mutual polarization and hydration effects, improving understanding of protein solution dielectric behavior.

Findings

Theory accurately predicts saccharide absorption without fitting parameters.

Experimental protein absorption exceeds standard models, indicating hydration shell polarization.

Using simulation data, the model reproduces the observed absorption peak in proteins.

Abstract

A theory of radiation absorption by dielectric mixtures is presented. The coarse-grained formulation is based on the wavevector-dependent correlation functions of molecular dipoles of the host polar liquid and a density-density structure factor of the positions of the solutes. A nonlinear dependence of the absorption coefficient on the solute concentration is predicted and originates from the mutual polarization of the liquid surrounding the solutes by the collective field of the solute dipoles aligned along the radiation field. The theory is applied to terahertz absorption of hydrated saccharides and proteins. While the theory gives an excellent account of the observations for saccharides without additional assumptions and fitting parameters, experimental absorption coefficient of protein solutions significantly exceeds theoretical calculations within standard dielectric models and shows a peak against the protein concentration. A substantial polarization of protein's hydration shell is required to explain the differences between standard theories and experiment. When the correlation function of the total dipole moment of the protein with its hydration shell from numerical simulations is used in the present analytical model an absorption peak similar to that seen is experiment is obtained. The result is sensitive to the specifics of protein-protein interactions in solution. Numerical testing of the theory requires the combination of terahertz dielectric and small-angle scattering measurements.