Pixel multiplexing for high-speed multi-resolution fluorescence imaging

TL;DR

This paper presents a novel pixel multiplexing technique that enables simultaneous high-resolution imaging and high-speed sequence acquisition by transiently masking subregions during a single CCD exposure, demonstrated in cardiac calcium imaging.

Contribution

The method introduces a new imaging modality that embeds temporal information within a single frame without increasing bandwidth, enabling high-speed multi-resolution imaging.

Findings

Achieved 250 Hz imaging of calcium transients in heart cells.

Demonstrated simultaneous high-resolution and high-speed imaging with a standard camera.

Validated the technique's effectiveness in biological imaging applications.

Abstract

We introduce a imaging modality that works by transiently masking image-subregions during a single exposure of a CCD frame. By offsetting subregion exposure time, temporal information is embedded within each stored frame, allowing simultaneous acquisition of a full high spatial resolution image and a high-speed image sequence without increasing bandwidth. The technique is demonstrated by imaging calcium transients in heart cells at 250 Hz with a 10 Hz megapixel camera.