Contractile prestress controls stiffening and fluidization of living cells in response to large external forces

TL;DR

This study investigates how living cells respond mechanically to large external forces, revealing that their stiffening and fluidization are governed by contractile prestress, with implications for cell-environment interactions.

Contribution

It demonstrates that cell mechanical responses under large forces are controlled by contractile prestress, providing a quantitative relationship and insights into cell-environment interactions.

Findings

Stress stiffening and fluidization occur simultaneously.

Cell prestress determines the extent of mechanical response.

Creep response follows a weak power law across force levels.

Abstract

We report the simultaneous characterization of time- and force-dependent mechanical properties of adherent cells in the physiologically relevant regime of large forces. We used magnetic tweezers to apply forces to magnetic beads bound to the cytoskeleton, and recorded the resulting deformation (creep response). The creep response followed a weak power law at all force levels. Stress stiffening and fluidization occurred simultaneously and were quantified by the force-dependence of the creep compliance and the power law exponent. The amount of stiffening and fluidization in response to force was controlled solely by the contractile prestress of the cell and followed a simple relationship. This behavior may be of fundamental importance in biological processes that involve a mechanical interaction between cells and their environment.