Hyperthermia HeLa cell treatment with silica coated manganese oxide nanoparticles

TL;DR

This study demonstrates that silica-coated manganese oxide nanoparticles can induce apoptosis in HeLa cells under a high-frequency magnetic field, achieving cell death without significant temperature increase, suggesting a promising hyperthermia treatment approach.

Contribution

The paper introduces a novel silica-coated manganese oxide nanoparticle for hyperthermia therapy that effectively induces apoptosis in cancer cells at low temperature increases.

Findings

Nanoparticles cause cell death via apoptosis under magnetic field.

Cell survival unaffected by nanoparticles or field alone.

Significant cell damage occurs with combined treatment despite minimal temperature rise.

Abstract

The effect of a high frequency alternating magnetic field on HeLa tumour cells incubated with ferromagnetic nanoparticles of manganese oxide perovskite La0.56(SrCa)0.22MnO3 have been studied. The particles were subjected to a size selection process and coated with silica to improve their biocompatibility. The control assays made with HeLa tumour cells showed that cell survival and growth rate were not affected by the particle internalization in cells, or by the electromagnetic field on cells without nanoparticles. However, the application of an alternating electromagnetic field to cells incubated with this silica coated manganese oxide induced a significant cellular damage that finally lead to cell death by an apoptotic mechanism. Cell death is triggered even thought the temperature increase in the cell culture during the hyperthermia treatment is lower than 0.5 C.