Terahertz Biomems for Enzymatic Catalysis Monitoring

TL;DR

This paper presents a BioMEMS device capable of monitoring enzymatic reactions using terahertz spectroscopy, demonstrating the feasibility of non-invasive, real-time biocatalysis analysis.

Contribution

It introduces a novel terahertz-compatible BioMEMS with enzyme immobilization and demonstrates its potential for enzymatic reaction monitoring.

Findings

Terahertz spectroscopy can detect enzymatic activity within microchannels.

The fabricated device successfully immobilizes enzymes for THz analysis.

Preliminary results confirm the feasibility of terahertz monitoring of biocatalysis.

Abstract

This work focuses on the fabrication and use of a Biological Micro-ElectroMechanical System (BioMEMS) for TeraHertz (THz) detection and characterization of enzymatic catalysis reactions. The fluidic microdevice was fabricated using traditional lithographic techniques. It was then functionalized by reactive amine functions using plasma polymerized allylamine (pp-allylamine), followed by enzyme immobilization inside the microchannels. The enzymatic reaction was controlled by fluorescent microscopy before being analysed by sub-THz spectroscopy in the frequency range 0.06--0.11 THz. The preliminary results show the feasibility of terahertz monitoring of the biocatalysis reaction.