Arbitrary multi-site two-photon excitation in four dimensions
Vincent Ricardo Daria, Christian Stricker, Richard Bowman, Stephen, Redman, Hans-A. Bachor
TL;DR
This paper presents a method for dynamic, arbitrary multisite two-photon excitation in three dimensions using holographic projection, enabling rapid, precise stimulation for biological, microfabrication, and holographic storage applications.
Contribution
It introduces a high-speed, noniterative hologram calculation technique for real-time multisite 3D two-photon excitation with potential for various advanced applications.
Findings
Achieved rapid, arbitrary multisite 3D two-photon excitation
Demonstrated sufficient photon density for localized excitation
Enabled applications in biological and microfabrication fields
Abstract
We demonstrate dynamic and arbitrary multisite two-photon excitation in three dimensions using the holographic projection method. Rapid response (fourth dimension) is achieved through high-speed noniterative calculation of the hologram using a video graphics accelerator board. We verify that the projected asymmetric spot configurations have sufficient spatiotemporal photon density for localized two-photon excitation. This system is a significant advance and can be applied to time-resolved photolysis of caged compounds in biological cells and complex neuronal networks, nonlinear microfabrication and volume holographic optical storage.
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