Enzymatic AND-Gate Based on Electrode-Immobilized Glucose-6-Phosphate Dehydrogenase: Towards Digital Biosensors and Biochemical Logic Systems with Low Noise

TL;DR

This paper demonstrates an enzymatic AND gate using electrode-immobilized glucose-6-phosphate dehydrogenase, achieving a sigmoid response and analyzing its potential for low-noise digital biosensing and biocomputing.

Contribution

It introduces a novel enzymatic AND gate with a sigmoid response and provides a kinetic model to evaluate its performance for biocomputing applications.

Findings

First experimental realization of sigmoid response in enzymatic AND gate

Kinetic model evaluates proximity to optimal gate performance

Potential for low-noise digital biosensors and biocomputing

Abstract

Electrode-immobilized glucose-6-phosphate dehydrogenase is used to catalyze an enzymatic reaction which carries out the AND logic gate. This logic function is considered here in the context of biocatalytic processes utilized for the biocomputing applications for "digital" (threshold) sensing/actuation. We outline the response functions desirable for such applications and report the first experimental realization of a sigmoid-shape response in one of the inputs. A kinetic model is developed and utilized to evaluate the extent to which the experimentally realized gate is close to optimal.