How accurate are polymer models in the analysis of Forster resonance energy transfer experiments on proteins?

TL;DR

This study evaluates the accuracy of common polymer models in interpreting FRET data on proteins, revealing that while mean distances are well-estimated, other parameters like Rg and persistence length are less reliable, and proposing a self-consistency test for model validation.

Contribution

The paper introduces a self-consistency test to evaluate the validity of polymer models in FRET analysis of protein DSEs, highlighting limitations of Gaussian models.

Findings

Mean end-to-end distance can be inferred with less than 10% error.

Radius of gyration and persistence length estimates can have up to 25% error.

Gaussian model inadequately describes the DSE P(R) in experimental data.

Abstract

Single molecule Forster resonance energy transfer (FRET) experiments are used to infer the properties of the denatured state ensemble (DSE) of proteins. From the measured average FRET efficiency, <E>, the distance distribution P(R) is inferred by assuming that the DSE can be described as a polymer. The single parameter in the appropriate polymer model (Gaussian chain, Wormlike chain, or Self-avoiding walk) for P(R) is determined by equating the calculated and measured <E>. In order to assess the accuracy of this "standard procedure," we consider the generalized Rouse model (GRM), whose properties [<E> and P(R)] can be analytically computed, and the Molecular Transfer Model for protein L for which accurate simulations can be carried out as a function of guanadinium hydrochloride (GdmCl) concentration. Using the precisely computed <E> for the GRM and protein L, we infer P(R) using the standard procedure. We find that the mean end-to-end distance can be accurately inferred (less than 10% relative error) using <E> and polymer models for P(R). However, the value extracted for the radius of gyration (Rg) and the persistence length (lp) are less accurate. The relative error in the inferred R-g and lp, with respect to the exact values, can be as large as 25% at the highest GdmCl concentration. We propose a self-consistency test, requiring measurements of <E> by attaching dyes to different residues in the protein, to assess the validity of describing DSE using the Gaussian model. Application of the self-consistency test to the GRM shows that even for this simple model the Gaussian P(R) is inadequate. Analysis of experimental data of FRET efficiencies for the cold shock protein shows that at there are significant deviations in the DSE P(R) from the Gaussian model.