Three-dimensional structure determination from a single view

TL;DR

This paper introduces ankylography, a novel 3D imaging method that reconstructs complete three-dimensional structures from a single 2D diffraction pattern, enabling rapid and potentially single-pulse 3D imaging of various materials.

Contribution

The paper presents the concept and experimental validation of ankylography, a new technique for 3D structure determination from a single diffraction pattern, differing from traditional multi-view or sectioning methods.

Findings

Successfully reconstructed 3D structures of a glass and a virus from single diffraction patterns.

Demonstrated feasibility using soft X-ray laser data for experimental validation.

Potential for rapid, single-shot 3D imaging in biological and disordered materials.

Abstract

The ability to determine the structure of matter in three dimensions has profoundly advanced our understanding of nature. Traditionally, the most widely used schemes for 3D structure determination of an object are implemented by acquiring multiple measurements over various sample orientations, as in the case of crystallography and tomography (1,2), or by scanning a series of thin sections through the sample, as in confocal microscopy (3). Here we present a 3D imaging modality, termed ankylography (derived from the Greek words ankylos meaning 'curved' and graphein meaning 'writing'), which enables complete 3D structure determination from a single exposure using a monochromatic incident beam. We demonstrate that when the diffraction pattern of a finite object is sampled at a sufficiently fine scale on the Ewald sphere, the 3D structure of the object is determined by the 2D spherical pattern. We confirm the theoretical analysis by performing 3D numerical reconstructions of a sodium silicate glass structure at 2 Angstrom resolution and a single poliovirus at 2 - 3 nm resolution from 2D spherical diffraction patterns alone. Using diffraction data from a soft X-ray laser, we demonstrate that ankylography is experimentally feasible by obtaining a 3D image of a test object from a single 2D diffraction pattern. This approach of obtaining complete 3D structure information from a single view is anticipated to find broad applications in the physical and life sciences. As X-ray free electron lasers (X-FEL) and other coherent X-ray sources are under rapid development worldwide, ankylography potentially opens a door to determining the 3D structure of a biological specimen in a single pulse and allowing for time-resolved 3D structure determination of disordered materials.