Organelle proteomics
Pierre Lescuyer, Mireille Chevallet (BBSI), Sylvie Luche (BBSI),, Thierry Rabilloud (BBSI)
TL;DR
This paper reviews strategies for organelle proteomics, focusing on isolating nuclei and analyzing their proteomes using targeted proteomics techniques, integrating previous methods and providing specific protocols.
Contribution
It offers a comprehensive overview of organelle proteomics methods and detailed protocols for nuclear isolation, advancing targeted proteomics research.
Findings
Protocols for nuclear isolation from various sources
Integration of existing proteomics techniques for organelle study
Guidelines for targeted organelle proteomics
Abstract
This unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided. Specific protocols for the isolation of nuclei from various sources (cell cultures, tissues) are also provided.
Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.