Simultaneous reconstruction of phase and amplitude contrast from a single holographic record

TL;DR

This paper introduces a method for reconstructing both phase and amplitude contrast from a single in-line hologram, demonstrated on optical and electron holograms, enabling detailed object characterization.

Contribution

It presents a novel reconstruction technique for simultaneous retrieval of absorption and phase properties from a single holographic record.

Findings

Successfully applied to optical holograms of micrometer objects

Extended to electron holograms of DNA molecules

Demonstrates accurate phase and amplitude reconstruction

Abstract

We present a reconstruction technique for simultaneous retrieval of absorption and phase shifting properties of an object recorded by in-line holography. The routine is experimentally tested by applying it to optical holograms of a pure phase respectively amplitude object of micrometer dimensions that has been machined into a glass-plate using a focused ion beam. The method has also been applied to previously published electron holograms of single DNA molecules.