Changes in chromatin state in donors subjected to physical stress

TL;DR

This study investigates how controlled physical stress affects chromatin structure in human buccal epithelial cells, revealing increased heterochromatinization following physical activity, with variability among individuals.

Contribution

It provides new insights into chromatin changes in human cells under physical stress, highlighting individual differences in response.

Findings

Physical stress increases heterochromatinization in buccal cells.

Heterochromatin levels rise after initial training, with some individuals showing significant increases after subsequent training.

Physiological indexes correlate with chromatin changes.

Abstract

The purpose of the present study is to evaluate changes in chromatin of human buccal epithelium under the influence of stressing factor - dosed physical activity. Investigations were performed in a group of students (13 men) of age 19-23. Cells were stained on a slide by a 2% orcein solution in 45% acetic acid during 1 h. The following physiological indexes were determined: arterial blood pressure, pulse frequency, and frequency of breathing. The physical stress produced by the dosed physical activity causes the considerable increase of degree of heterochromatinization in the cell nuclei of human buccal epithelium. As a rule, the level of heterochromatinization increases after first stage of training, but in some donors it increases significantly only after the second stage of training.