Carbon Nanotubes in Biology and Medicine: in vitro and in vivo Detection, Imaging and Drug Delivery

TL;DR

This review discusses the unique properties of carbon nanotubes and their applications in biological detection, imaging, and drug delivery, emphasizing surface functionalization for biocompatibility and effectiveness.

Contribution

It provides a comprehensive summary of recent advances in CNT-based biomedical applications, highlighting the importance of surface functionalization and demonstrating various detection and imaging techniques.

Findings

Surface functionalization enhances biocompatibility and detection sensitivity.

CNT-based sensors enable label-free, ultra-sensitive biological detection.

CNTs show promise as drug delivery vehicles and imaging contrast agents.

Abstract

Carbon nanotubes exhibit many unique intrinsic physical and chemical properties and have been intensively explored for biological and biomedical applications. In this review, we summarize the main results of our and other groups in this field and clarify that surface functionalization is critical to the behaviors of carbon nanotubes in biological systems. Ultra-sensitive detection of biological species with carbon nanotubes can be realized after surface passivation to inhibit the non-specific binding of bio-molecules on the hydrophobic nanotube surface. Electrical nanosensors based on nanotubes provide a label-free approach to biological detections. Surface enhanced Raman spectroscopy of CNT opens up a method of protein microarray with down to 1 fM detection sensitivity. In vitro and in vivo toxicity studies reveal that well water soluble and serum stable nanotubes are biocompatible, non-toxic and potentially useful for biomedical applications. In vivo biodistributions vary with the functionalization and possibly also sizes of nanotubes, with a tendency of accumulation in the reticuloendothelial systems, including the liver and spleen, after intravenous administration. If well functionalized, nanotubes may be excreted mainly through the biliary pathway in feces. Carbon nanotube-based drug delivery has shown promises in various in vitro and in vivo experiments including delivery pf small interfering RNA, paclitaxel and doxorubicin. Moreover, SWNTs with various interesting intrinsic optical properties have been used as novel photoluminance, Raman and photoacoustic contrast agents for imaging of cells and animals. Further multidisciplinary explorations in this field are promising and may bring new opportunities to the realm of biomedicine.