Label-free electrical quantification of the dielectrophoretic response of DNA
Anja Henning, Joerg Henkel, Frank F Bier, Ralph Hoelzel
TL;DR
This paper introduces a novel electrical sensing method to quantify DNA concentration and dielectrophoretic response without chemical modifications, achieving high sensitivity and potential single-molecule detection.
Contribution
It presents a label-free electrical technique to measure DNA dielectrophoretic response and concentration, avoiding chemical modifications and enabling high sensitivity.
Findings
DNA concentrations in the ng/mL range measured
Electrical parameters influence dielectrophoretic response
Good agreement with fluorescently labelled DNA data
Abstract
A purely electrical sensing scheme is presented that determines the concentration of macromolecules in solution by measuring the capacitance between planar microelectrodes. Concentrations of DNA in the ng/mL range have been used in samples of 1 microL volume. The method has been applied to the characterisation of the dielectrophoretic response of DNA without the need for any chemical modifications. The influence of electrical parameters like duty cycle, voltage and frequency has been investigated. The results are in good agreement with data from dielectrophoretic studies on fluorescently labelled DNA. Extension of the method down to the single molecule level appears feasible.
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