The Dynamic Replicon: adapting to a changing cellular environment

TL;DR

This paper reviews how eukaryotic cells dynamically adapt DNA replication origins in response to changing cellular environments and developmental cues, highlighting mechanisms of origin selection and regulation.

Contribution

It provides an overview of recent evidence on the mechanisms regulating replication origin selection and adaptation during normal and stressed cell cycles.

Findings

Replication origins are specified by multiple potential sites.

Origin selection is influenced by cellular conditions and developmental stage.

Replication patterns are highly adaptable to environmental and internal changes.

Abstract

Eukaryotic cells are often exposed to fluctuations in growth conditions as well as endogenous and exogenous stress-related agents. In addition, during development global patterns of gene transcription change dramatically, and these changes are associated with altered patterns of DNA replication. In metazoan embryos, for example, transcription is repressed globally and any sequence in the genome can serve as a site for the start of DNA synthesis. As transcription is activated and a G1 phase imposed, the pattern of replication adapts to these changes by restricting the sites where DNA synthesis begins. Recent evidence indicates that each unit of replication, or replicon, is specified by two or more potential replication origins, but only one is selected to initiate replication of the replicon. How the cell distinguishes between potential origins, and how it selects a given origin of replication remain unclear. This raises important questions concerning the nature and definition of the eukaryotic replicon. In the following we will review emerging evidence concerning the mechanisms involved in regulating replication origins during both the normal and perturbed eukaryotic cell cycle.