Determination of volume, shape and refractive index of individual blood platelets

TL;DR

This study combines theoretical modeling and experimental analysis of light scattering patterns to determine the volume, shape, and refractive index of individual blood platelets, using simplified spheroid models and the T-matrix method.

Contribution

It introduces a simplified optical model for blood platelets and applies the T-matrix method to accurately characterize their physical properties from light scattering data.

Findings

Successful determination of platelet volume, shape, and refractive index distributions.

Validation of the spheroid model for blood platelet scattering patterns.

Demonstrated the effectiveness of the T-matrix method in biological particle analysis.

Abstract

Light scattering patterns (LSP) of blood platelets were theoretically and experimentally analyzed. We used spicular spheroids as a model for the platelets with pseudopodia. The discrete dipole approximation was employed to simulate light scattering from an individual spicular spheroid constructed from a homogeneous oblate spheroid and 14 rectilinear parallelepipeds rising from the cell centre. These parallelepipeds have a weak effect on the LSP over the measured angular range. Therefore, a homogeneous oblate spheroid was taken as a simplified optical model for platelets. Using the T-matrix method, we computed the LSP over a range of volumes, aspect ratios and refractive indices. Measured LSPs of individual platelets were compared one by one with the theoretical set and the best fit was taken to characterize the measured platelets, resulting in distributions of volume, aspect ratio and refractive index.