Immobilization of single strand DNA on solid substrate

TL;DR

This paper demonstrates a method for immobilizing single strand DNA on solid substrates using Layer-by-Layer films with polycation PAH, maintaining stability and organization even after temperature changes.

Contribution

It introduces a novel approach for immobilizing single strand DNA on solid supports via electrostatic interaction in LbL films, especially above DNA melting temperature.

Findings

DNA remains immobilized after temperature cycling

DNA organization remains stable over several days

UV-Vis confirms successful immobilization

Abstract

Thin films based on Layer-by-Layer (LbL) self assembled technique are useful for immobilization of DNA onto solid support. This communication reports the immobilization of DNA onto a solid support by electrostatic interaction with a polycation Poly (allylamine hydrochloride) (PAH). UV-Vis absorption and steady state fluorescence spectroscopic studies exhibit the characteristics of DNA organized in LbL films. The most significant observation is that single strand DNA are immobilized on the PAH backbone of LbL films when the films are fabricated above the melting temperature of DNA. DNA immobilized in this way on LbL films remains as such when the temperature is restored at room temperature and the organization remains unaffected even after several days. UV-Vis absorption spectroscopic studies confirm this finding.