Influence of DNA on Ag(I)-C-Phycocyanin complexes

TL;DR

This study investigates how DNA influences the fluorescence behavior of silver phycocyanin complexes, revealing interactions that affect fluorescence intensity and spectral shifts, with potential implications for understanding biomolecular interactions.

Contribution

It provides new insights into DNA's role in modulating the fluorescence of silver phycocyanin complexes through spectroscopic analysis.

Findings

DNA addition restores fluorescence after silver-induced quenching

Silver binding causes blue shifts in fluorescence spectra

Quantitative Stern-Volmer quenching constants were determined

Abstract

The effects of DNA on the fluorescence of silver phycocyanin complexes were investigated. It was shown, that the fluorescence intensity of phycocyanin decreases, when silver ions are added but the following DNA addition gives rise to fluorescence buildup. Fluorescence spectroscopy provides insight of silver phycocyanin interactions. Fluorescence measurements demostrate phycocyanin quenching of silver emission intensities. SternVolmer quenching constant was obtained from the linear quenching plot. Blue shifts in the fluorescence spectra were observed during silver binding to phycocyanin.