A three-state kinetic mechanism for scaffold mediated signal transduction

TL;DR

This paper develops a minimal three-state kinetic model to understand how scaffold proteins influence kinase activation timing and signaling dynamics in eukaryotic cells, supported by analytical and numerical solutions.

Contribution

It introduces a path summation technique for approximate solutions and analyzes the effects of scaffold proteins on signal transduction timing.

Findings

Model aligns with experimental data

Scaffold proteins broaden kinase activation times

Provides a framework for understanding signal timing

Abstract

Signaling events in eukaryotic cells are often guided by a scaffolding protein. Scaffold proteins assemble multiple proteins in a spatially localized signaling complex and exert numerous physical effects on signaling pathways. To study these effects, we consider a minimal, three-state kinetic model of scaffold mediated kinase activation. We first introduce and apply a path summation technique to obtain approximate solutions to a single molecule master equation that governs protein kinase activation. We then consider exact numerical solutions. We comment on when this approximation is appropriate and then use this analysis to illustrate the competition of processes occurring at many time scales involved in signal transduction in the presence of a scaffold protein. The findings are consistent with recent experiments and simulation data. Our results provide a framework and offer a mechanism for understanding how scaffold proteins can influence the shape of the waiting time distribution of kinase activation and effectively broaden the times over which protein kinases are activated in the course of cell signaling.