Binding Isotherms and Cooperative Effects for Metal-DNA Complexes

TL;DR

This study investigates the binding constants and cooperative effects of various metal ions with DNA from Spirulina platensis, revealing different interaction mechanisms and correlations with covalent indices.

Contribution

It provides new quantitative data on metal-DNA interactions and identifies distinct cooperative behaviors for different metal ions.

Findings

Cobalt(II) and Zinc(II) show cooperative effects with DNA.

Nickel(II) and Silver(I) form independent binding sites.

Negative cooperativity observed for Manganese(II).

Abstract

The stoichiometric binding constants of Nickel(II), Cobalt(II), Manganese(II), Silver(I), Zinc(II) ions with DNA, from Spirulina platensis were determined from their binding isotherms by equilibrium dialysis and atomic absorption spectroscopy. It was shown, that the nature of these ions interaction with DNA, from S .platensis is different. For Cobalt(II), Zinc(II) ions were observed cooperative effects and existence of two different types of the binding sites. Nickel(II)_, Silver(I) -DNA complexes shows independent and identical binding sites and Manganese(II)_ negative cooperative interaction. The logarithm of binding constants for Cobalt (II)_, Nickel (II)_, Manganese (II)_, Zinc (II)_, Silver (I) - DNA, from S. platensis in 3 mM Na(I) are 5.11; 5.18; 4.77; 5.05; 5.42; respectively. The linear correlation of logarithm of binding constants (for complexes of metal-DNA from S. platensis) and the covalent index of Pauling are observed.