Transcriptional bursts: a unified model of machines and mechanisms

TL;DR

This paper introduces a comprehensive model of transcriptional bursts that integrates the mechano-chemistry of RNA polymerases, their interactions, and gene state switching, explaining burst statistics and how to control them.

Contribution

It presents a unified model combining molecular machine dynamics, interactions, and gene regulation to explain transcriptional bursting phenomena.

Findings

The model captures the statistical properties of transcriptional bursts.

It demonstrates how burst features can be tuned by controlling RNAP steps.

The model links molecular mechanisms to observable burst patterns.

Abstract

{\it Transcription} is the process whereby RNA molecules are polymerized by molecular machines, called RNA polymerase (RNAP), using the corresponding DNA as the template. Recent {\it in-vivo} experiments with single cells have established that transcription takes place in "bursts" or "pulses". In this letter we present a model that captures not only the mechano-chemistry of individual RNAPs and their steric interactions but also the switching of the gene between the ON and OFF states. This model accounts for the statistical properties of the transcriptional bursts. It also shows how the quantitative features of the distributions of these bursts can be tuned by controlling the appropriate steps of operation of the RNAP machines.