A Solid-State System for High Temporal Resolution Fluorescence Lifetime Measurements
M. Gersbach, D. L. Boiko, C. Niclass, C. Petersen, E. Charbon
TL;DR
This paper introduces a CMOS-based solid-state system capable of high temporal resolution fluorescence lifetime measurements, enabling detailed analysis of rapid fluorescence decay in calcium indicators with unprecedented precision.
Contribution
The work presents a novel CMOS single photon avalanche diode system achieving 79 ps response time for fluorescence lifetime measurements in the two-photon regime.
Findings
Achieved an instrument response function of 79 ps.
Successfully modeled fluorescence decay with a triple exponential fit.
Demonstrated high temporal resolution in fluorescence lifetime analysis.
Abstract
A system for time-correlated single photon counting applications based on CMOS single photon avalanche diodes is presented. An instrument response function of 79 ps allows a fluorescence decay of high-affinity Ca2+ indicator Oregon Green BAPTA-1 to be analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay model is shown to best fit the fluorescence dynamics of OGB-1.
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Taxonomy
TopicsAdvanced Optical Sensing Technologies · Advanced Fluorescence Microscopy Techniques · CCD and CMOS Imaging Sensors