Compound-specific isotope analysis

TL;DR

This paper reviews the development and applications of compound-specific isotope analysis, a technique that measures isotopic compositions at the molecular level to study biogeochemical processes in complex mixtures.

Contribution

It provides a comprehensive overview of recent advances and diverse applications of compound-specific isotope analysis across scientific disciplines.

Findings

Isotope analysis can trace biogeochemical transformations.

Molecular-level techniques enable trace analysis in complex samples.

Applications span environmental, biological, and geological sciences.

Abstract

The isotopic composition, for example, 14C/12C, 13C/12C, 2H/1H, 15N/14N and 18O/16O, of the elements of matter is heterogeneous. It is ruled by physical, chemical and biological mechanisms. Isotopes can be employed to follow the fate of mineral and organic compounds during biogeochemical transformations. The determination of the isotopic composition of organic substances occurring at trace level in very complex mixtures such as sediments, soils and blood, has been made possible during the last 20 years due to the rapid development of molecular level isotopic techniques. After a brief glance at pioneering studies revealing isotopic breakthroughs at the molecular and intramolecular levels, this paper reviews selected applications of compound-specific isotope analysis in various scientific fields.