Small Angle Scattering and Zeta Potential of Liposomes Loaded with Octa(carboranyl)porphyrazine

TL;DR

This study characterizes liposomes loaded with a novel boron-containing compound using small angle scattering and zeta potential measurements, providing insights into their structure and surface charge for potential anticancer therapies.

Contribution

It introduces a detailed physicochemical analysis of liposomes loaded with a new carboranyl porphyrazine, combining structural and surface charge data to understand their properties.

Findings

Liposomes showed specific size and charge characteristics depending on loading methods.

Structural analysis revealed differences in liposome organization based on lipid composition.

Surface charge measurements correlated with liposome stability and loading efficiency.

Abstract

In this work the physicochemical characterization of liposomes loaded with a newly synthesised carboranyl porphyrazine (H2HECASPz) is described. This molecule represents a potential drug for different anticancer therapies, such as Boron Neutron Capture Therapy, Photodynamic Therapy and Photothermal Therapy. Different loading methods and different lipid mixtures were tested. The corresponding loaded vectors were studied by Small Angle Scattering (SANS and SAXS), light scattering and zeta potential. The combined analysis of structural data at various length scales and the measurement of the surface charge allowed to obtain a detailed characterization of the investigated systems. The mechanisms underlying the onset of differences in relevant physicochemical parameters (size, polydispersity and charge) were also critically discussed.