Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy

TL;DR

This study uses two-photon microscopy to observe NAD(P)H autofluorescence in macrophages under various conditions, revealing differences in morphology and intensity that could inform understanding of atherosclerosis.

Contribution

It demonstrates the application of two-photon microscopy for detailed autofluorescence imaging of macrophages on different surfaces, highlighting optimal conditions for observing cellular profiles.

Findings

Fibronectin coating yields the best macrophage autofluorescence images.

Distinct autofluorescence intensities and morphologies are observed under different surface conditions.

Two-photon microscopy provides a low-damage, high-contrast method for macrophage imaging.

Abstract

Ox-LDL uptakes by macrophage play a critical role in the happening of atherosclerosis. Because of its low damage on observed cells and better signal-to- background ratio, two-photon excitation fluorescence microscopy is used to observe NAD(P)H autofluorescence of macrophage under difference cultured conditions- bare cover glass, coated with fibronectin or poly-D-lysine. The results show that the optimal condition is fibronectin coated surface, on which, macrophages profile can be clearly identified on NAD(P)H autofluorescence images collected by two-photon microscopy. Moreover, different morphology and intensities of autofluorescence under different conditions were observed as well. In the future, effects of ox-LDL on macrophages will be investigated by purposed system to research etiology of atherosclerosis.